Author/Authors :
Hakemi-Vala، Mojdeh نويسنده Department of Microbiology, Medical School , , Mehrara، Mina نويسنده 2 Department of Microbiology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran , , Pourramezan، Mojgan نويسنده 2 Department of Microbiology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran , , Asgarpanah، Jinous نويسنده Pharmacognosy Department, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran-Iran , , Rahimifard ، Nahid نويسنده Department of Microbiology; Food and Drug Control Laboratories; Food And Drug Laboratories Research Center; Ministry of Health and Medical Education, , , Khoshnood، Saeed نويسنده Department of Microbiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Heidary، Mohsen نويسنده Depatrment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,
Abstract :
Background: With increasing use of antibiotics, the number and type of microbial resistance to antibiotics have been raised and at result the need for synthesis of new antimicrobials or acquire new sources of antimicrobial is indisputable. In this study the antimicrobial effects of the flowering aerial parts of Glaucium vitellinum and Gaillonia aucheri were investigated.
Materials and Methods: The antimicrobial effect of mentioned extracts against Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1430, Salmonella typhimurium PTCC 1639, Staphylococcus aureus PTCC 1431 and Candida albicans PTCC 5027 were evaluated by disk diffusion and the microdilution method based on CLSI protocol 2012.
Results: No any zone of inhibition were detected by disk diffusion method against tested microbes for Glaucium vitellinum. After deletion of agar interference, the minimal inhibitory concentration (MIC) was determined by broth microdilution method for two plants. None of tested extracts were effective against Gram negative tested bacteria except alkaloid fraction of G. vitellinum which was effective against S. typhi. In contrast, the extracts and fractions of both plans were effective against tested gram positive bacteria especially S. aureus.
Conclusion: The best result of MIC was detected for alkaloid fraction of G. vitellinum (0.09 mg/ml) Vs. G. aucheri (125 mg/ml) against S.aureus.
