Novel Cationic Dye Based on Naphthalimide: Part 1: Synthesis, Characterization and Evaluation of Biology Efficacy as Antimicrobial Agent

In this paper, synthesis and antimicrobial properties of cationic dye were investigated. 4-acetylamino-N-2-aminomethylpyridine-1,8-naphthalimide was reacted with 1-bromobutane as the alkylating agent and a cationic dye was obtained. The final product was purified by column chromatography method. The chemical structure of the novel cationic dye and its intermediates was chracterisized by using FTIR, 1HNMR, 13CNMR and DSC techniques. The antimicrobial efficacy of the dye was evaluated by using a minimum inhibitory cocentration MIC and disk difusion methods. The synthesized dye and its intermediates showed good antimicrobial activities against both Gram positive and Gram negative bacteria. Also, the results indicated that cationic dye 3 containing 1-bromobutane as the alkylating agent had highest antimicrobial activity against all the bacteria used.The MIC of the novel cationic dye against E. coli was 125 μg ml−1 and S.aureus was 62.5 μg ml−1.