Author/Authors :
Tajik، Parviz نويسنده , , Kohsari، Hesam نويسنده Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. , , Qasemi-Panahi، Babak نويسنده Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. , , Sohrabi-Haghdoost، Iraj نويسنده Department of Pathology, Faculty of Veterinary Medicine, Science and Research¬ Branch, Islamic Azad University, Tehran, Iran. , , Barin، Abbas نويسنده Department of Clinical Pathology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran Barin, Abbas , Ghasemzadeh Nava، Hamid نويسنده Clinical Science Department, Faculty of Veterinary Medicine , University of Tehran, Tehran, Iran ,
Abstract :
Identification of exogenous factors affecting spermatogonial stem cells (SSCs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. The aim of this study was to investigate the effects of a GnRH analogue (alareline acetate) on SSCs colonization in short-term co-culture with sertoli cells. Five, three-month old Holstein male calves were used to isolate spermatogonial and sertoli cells. Testicular germ cell collection was made by enzymatic digestion methods. The cells were co-cultured in a 15 day period and in vitro effects of various doses (0.5, 1, 2 and 4 µg/ml) of GnRHa on SSCs colonization were assessed. Effects of GnRHa on SSCs proliferation were dose dependent. In conclusion, it was demonstrated that 1 µg/ml GnRHa was the optimum dose for SSCs colonization in comparison with control group. The highest treatment dose (4 µg/ml GnRHa), negatively affected SSCs colonization in comparison with control group.
