Author/Authors :
Hatam Gholam Reza نويسنده , Babaei Bazkiyaei Zahra نويسنده , TAVAKOLI KARESHK Amir نويسنده Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran TAVAKOLI KARESHK Amir , Ziaali Naser نويسنده Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran. Ziaali Naser , TAVAKOLI OLIAEE Razieh نويسنده Dept. of Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran TAVAKOLI OLIAEE Razieh , MAHMOUDVAND Hosein نويسنده Dept. of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran MAHMOUDVAND Hosein , VAFAFAR Arghavan نويسنده Dept. of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran VAFAFAR Arghavan
Abstract :
Background: The present study aimed to characterize genetically and to compare the most frequently occurring strains of Trichomonas vaginalis isolated from southern Iran.
Methods: Totally, 150 vaginal swab and urine specimens were collected from symptomatic and asymptomatic women from May 2012 to Jun 2013.This study implemented a sensitive and reliable PCR-restriction fragment length polymorphism (RFLP) typing method on the actin gene. Moreover, one representative sample of each identified genotype was subjected to sequencing.
Results: Twenty-four T. vaginalis isolates were positive and6 distinct electrophoretic patterns (H, E, G, I, M, N) were identified. Genotypes H and I were found to be more prevalent (50 and 37.5%) in Kerman and Shiraz, respectively. The phylogenetic analysis showed that two isolates were located as a separated clade with the other T. vaginalis isolates.
Conclusion: The obtained findings showed a considerable genetic polymorphism of clinical isolates from the population studied. More studies may be warranted in future as to unveiling any possible links between a given genotype/cluster and pathogenic behavior of T. vaginalis.
