Author/Authors :
Rezaei Nima نويسنده , Mohammadzadeh Iraj نويسنده , MANSOURI MAHBOUBEH نويسنده , Mesdaghi Mehrnaz نويسنده Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences , Babaie Delara نويسنده Department of Allergy and Clinical Immunology, Hazrate Rasool Hospital, Tehran University of Medical Sciences , Alimohammadi Reza نويسنده Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , Rahimi Arezou نويسنده Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , Amirmoini Mehrdad نويسنده Department of Allergy and Clinical Immunology, Mofid Childrens Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran Amirmoini Mehrdad , Farajifard Hamid نويسنده 4Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran , Chavoshzadeh Zahra نويسنده 2Department of Allergy and Clinical Immunology, Mofid Childrens Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Background and Aims: Patients with Hyper-IgE syndrome suffer from fungal and bacterial infections, especially Candida albicans and Staphylococcus aureus. Due to the important role of T helper17 (Th17) lymphocytes in defense against fungal infections, the percentage of Th17 lymphocytes was studied in the patients with autosomal recessive hyper-IgE syndrome (AR-HIES).
Materials and Methods: In this case-control study, six patients with AR-HIES (with DOCK-8 mutation) and seven healthy age and sex-matched controls were included. Peripheral blood mononuclear cells were isolated from their venous blood and the percentage of Th17 lymphocytes were determined by flow cytometry.
Results: There was no statistical difference between the percentage of Th17 lymphocytes (p=0.15) in the case and control groups. Also in comparison to the control subjects, the numbers of eosinophils were dramatically increased (p=0.000). Also, there was a significant negative correlation between serum IgE levels and Th17 lymphocytes’ percentage (r=-0.927, p=0.006) and a significant positive correlation between eosinophils number and Th17 lymphocytes’ percentage (r=0.557, p=0.01). Serum IgE levels showed a significant positive correlation with the numbers of eosinophils in the patients’ peripheral blood with AR-HIES (r=0.961, p=0.003).
Conclusions: The numbers of Th17 in the patients with AR-HIES may not show statistical differences between the cases and controls. The numbers of eosinophils significantly increased in the patients AR-HIES compare to the controls.
