Author/Authors :
Anvari Morteza نويسنده , Yadegari Maryam نويسنده Department of Anatomical Sciences, Cellular and Molecular Research Center (CMRC), Faculty of Medicine, Ahvaz Jundishapur University of Medical Science , Nahangi Hossein نويسنده Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Nahangi Hossein , Astani Akram نويسنده Shahid Sadoughi University of Medical Sciences, Yazd, Iran AND Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany. Astani Akram , Nayeb zadeh Vajiheh نويسنده 1Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract :
Background and Aims: Chondrocytes and their differentiation play a central role in joint diseases. Effect of the transforming growth factor (TGF)-B1 on chondrocyte characteristics and differentiation is not clearly understood. This study was undertaken to investigate the effects of
TGF-B1 on tissue characteristics and morphology of chondrocytes against degradation induced by interleukin (IL)-l? in bovine nasal cartilage (BNC) explant culture.
Materials and Methods: BNC explants were cultured in DMEM and samples were divided into four groups. In group A (control); samples were only cultured in DMEM and group B; was treated with IL-1? (10 ng/ml), group C; treated with TGF-B1 (10 ng/ml) and group D; treated with IL-1? (10 ng/ml) + TGF-B1 (10 ng/ml) for 14 days. At days 3, 7 and 14 the media were collected and replaced with fresh media. Then, samples were harvested on days 3, 7 and 14 and chondrocyte morphology was assessed by invert microscopy. We used Massonʹs Trichrome stain to visualize collagen distribution and synthesis, and Safranin O and Alician blue to highlight the proteoglycan content.
Results: In the presence of IL-l?, most of the chondrocytes were transformed into fibroblast-like morphology with pyknotic nuclei at day 14 and proteoglycan and collagen in extracellular matrix (ECM) were destructed at this time. In the presence of TGF-B1, chondrocytes preserved their ordinary normal features. Also, TGF-B1 inhibited collagen and proteoglycan destruction and cartilage ECM showed normal characteristics.
Conclusions: IL-1? induced significant morphological changes in chondrocytes and increased the destruction of ECM. TGF-B1 could strongly preserves cartilage from IL-1? degradation effects.
