Author/Authors :
Somi Mohammad-Hossein نويسنده , Safaralizadeh Reza نويسنده Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Safaralizadeh Reza , Basiri Zeinab نويسنده Department of Animal Biology, Faculty of Natural Sciences,
University of Tabriz, Tabriz, Iran , Jabbarpour Bonyadi Morteza نويسنده Department of Animal Biology, Faculty of Natural Sciences,
University of Tabriz, Tabriz, Iran , Abdolmohammadi Reza نويسنده Department of Animal Biology, Faculty of Natural Sciences,
University of Tabriz, Tabriz, Iran
Abstract :
Background Transmission of Helicobacter pylori
occurs within families. It is known that elimination of H.
pylori may reduce the risk of peptic ulcer disease (PUD) and
gastric cancer (GC) and increase the risk of gastro-esophageal reflux
(GERD), Barrett’s esophagus, and esophageal adenocarcinoma. Objectives
The present study aimed to determine gastric cancer-related strains of
H. Pylori by precise diagnostic methods in patients
with gastritis and PUD in East Azerbaijan, Iran, where the incidence of
GC and esophageal cancer is high. Methods The PCR assay was performed on
the same biopsy samples used for Campylobacte-like
organism test (CLO test), using the vacA d region (a
gene predictor of gastric adenocarcinoma and peptic ulcer disease) and
16S rDNA gene in patients referring to the department
of endoscopy at Imam Reza hospital in Tabriz, Iran. This study was
conducted on individuals who had not received non-steroidal
anti-inflammatory drugs or any anti-Helicobacter therapy at least for 3
months prior to endoscopy. The collected data were analyzed using SPSS
version 19. Results The rate of false negative results by CLO test was
8.3%. However, no false-positive results were found by CLO test. Among
those patients infected with H. pylori,
vacA d1 -positive strains, one case had a history of
gastric cancer and another one had a history of gastric ulcer in
first-degree relatives. However, no family history of gastric cancer or
gastric ulcer was found in the patients with strains carrying the
vacA d2 genotype. Conclusions This study suggests
that the vacA PCR can be very useful in diagnosis,
treatment, and follow-up of patients with gastritis and PUD. The PCR
assay and CLO test can complement and confirm each other in
identification and eradication of cancer-related strains of H.
pylori. The exclusion of patients with suspected achlorhydria
from the study is likely a reason for the lack of false positive results
in CLO test.
