Author/Authors :
Mortazavi Seyed-Hossein نويسنده , Khosravi Mojtaba نويسنده , Ghasemi Zeinab نويسنده Department of Mycology, Health School, Tehran University of Medical Sciences, Tehran, IR Iran , Assadi Mehrdad نويسنده Department of Microbiology, Faculty of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, IR Iran Assadi Mehrdad , Hashemi Seyed Jamale نويسنده Department of Medical Parasitology and Mycology, School of
Public Health, National Institute of Health Research, Tehran
University of Medical Sciences, Tehran, IR Iran , Rezaei Sassan نويسنده Department of Medical Parasitology and Mycology, School of
Public Health, National Institute of Health Research, Tehran
University of Medical Sciences, Tehran, IR Iran , Kordbache Parivash نويسنده Department of Medical Parasitology and Mycology, School of
Public Health, National Institute of Health Research, Tehran
University of Medical Sciences, Tehran, IR Iran , Hosseinpoor Susan نويسنده Department of Medical Mycology and Parasitology,
Mazandaran University of Medical Sciences, Sari, IR
Iran , Abbastabar Mehdi نويسنده Department of Medical Mycology and Parasitology,
Mazandaran University of Medical Sciences, Sari, IR
Iran , Shamohammadi Safar نويسنده Department of Dermatology, School of Medicine, Razi
Hospital, Tehran University of Medical Sciences, Tehran, IR
Iran
Abstract :
Background Candida species are known as the
most common fungal pathogens isolated from clinical specimens that can
develop complications ranging from cutaneous to systemic diseases.
Objectives The present study was conducted to evaluate the diversity and
distribution of Candida species in various kinds of
cutaneous candidiasis in Tehran, Iran. In addition, the critical effects
of several predisposing factors on the induction and progression of the
disease were considered. Methods A total of 3000 samples were taken from
March 2014 to 2015. The samples were examined using direct microscopy
and culturing method. The polymorphism analysis was performed by using
polymerase chain reaction - restriction fragment length polymorphism
(PCR-RFLP) technique. The internal spacer region (ITS) of the fungal
rRNA genes was recruited for PCR amplification of target sequences and
Msp1 enzyme was employed to digest PCR amplicons. Results Out of 3000
samples, yeast was recovered in 290 (9.67%) cases. Onychomycosis was
observed predominantly in 164 nail samples (56.5%). The majority of
patients were within the age range of 51 - 60 years (22.14%). Women
working at home (housewives) showed the highest percentage of people at
risk (n = 135, 46.5%). Among patients with underlying diseases, diabetic
patients with 46 cases (14.2 %) had the highest susceptibility to
candidiasis. Genotypic identification showed that Candida
albicans is the most common species (n = 132, 45.5 %)
recovered from clinical samples, followed by C.
parapsilosis (n = 77, 26.5%), C. glabrata
(n = 22, 7.5 %), C. krusei (n = 16, 5.5 %),
C. tropicalis (n = 37, 12.7 %), and C.
guilliermondii (n = 6, 2%). Conclusions The results of the
present study, compared to the previous studies, showed a significantly
lower prevalence of cutaneous candidiasis. Among
Candida species, C. albicans was
still the most common infectious agent isolated from clinical samples.
Shifting toward non-albicans Candida species was not
confirmed in this study. In addition, our study revealed that there is a
direct correlation between some predisposing factors including age of
patients, and existence of some non-infectious diseases (e.g. metabolic
disorder) and occurrence of Candida infection.
