Title of article :
Molecular Characterization and Distribution of Class 1 Integron-Bearing
Methicillin Resistant Staphylococcus aureus Strains in Burn
Patients, Tehran, Iran
Author/Authors :
Fazeli Maryam نويسنده Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , Goudarzi Mehdi نويسنده Department of Pharmacology and Toxicology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Goudarzi Mehdi , Seyedjavadi Sima Sadat نويسنده Research center for gastroenterology and liver diseases, Shahid Beheshti University of Medical Sciences, Tehran , Goudarzi Hossein نويسنده Shahid Beheshti University of Medical Sciences , E Udo Edet نويسنده Departments of Microbiology, Faculty of Medicine, Kuwait
University, Safat 13110, Kuwait , Beiranvand Elham نويسنده Protein Chemistry Unit, Biotechnology Research Center,
Pasteur Institute of Iran, Tehran, Iran
Issue Information :
ماهنامه با شماره پیاپی سال 2017
Abstract :
Background Methicillin resistant Staphylococcus
aureus (MRSA) is an increasingly common hospital pathogen in
burn patients, which is known to cause over 50% of burn related deaths.
One of the serious threats associated with clinical isolates of MRSA is
multi-drug resistance, which is associated with integrons. Objectives
The aim of this study was to determine the distribution and molecular
types of MRSA in burn patients and their carriage of integrons. Methods
During a 7-month period, 106 MRSA isolates were collected from burn
wounds of patients admitted to a referral burn hospital in Tehran.
Antimicrobial susceptibility testing (AST) was performed for 12
antimicrobial agents. Polymerase chain reaction (PCR) was used to detect
nucA, mecA, pvl
and tsst-1 genes, and class 1 and 2 integrons. Multiplex PCR technique
was used to determine the Staphylococcal cassette chromosome
mec (SCCmec) types of MRSA
strains. All isolates were genotyped by staphylococcal protein A
(spa) typing. Results AST showed the lowest rate of
resistance to quinupristin-dalfopristin (19.8%), mupirocin (31.3%), and
rifampicin (37.7%). All isolates were susceptible to vancomycin,
teicoplanin, and linezolid. Multi-drug resistance was observed in 97% of
isolates. The most SCCmec type was
SCCmec type III (98.1%) while only 2 (1.9%) MRSA
isolates harbored SCCmec type IV.
SCCmec types I, II, and V were not detected. The
study revealed the presence of class 1 integron in 58 (54.7%) isolates
and class 2 integron in 3.8% of isolates. Six different
spa types of t030 (66%), t037 (14.2%), t065 (9.4%),
t1358 (4.7%), t937 (3.8%), and t084 (1.9%) were identified amongst the
isolates. Conclusions The study revealed a high prevalence of multi-drug
resistance (MDR), class 1 integron, SCCmec type III,
and spa type t030 amongst MRSA associated with burn
wounds in an Iranian hospital. The existence of
SCCmec type III in burn patients emphasizes the
nosocomial origin of these strains.
Journal title :
Astroparticle Physics
