Author/Authors :
Sai, Na Department of Nutrition and Food Hygiene - School of Public Health - Tianjin Medical University, Tianjin, People’s Republic of China , Sun, Wenjing Department of Nutrition and Food Hygiene - School of Public Health - Tianjin Medical University, Tianjin, People’s Republic of China , Wu, Yuntang Department of Nutrition and Food Hygiene - School of Public Health - Tianjin Medical University, Tianjin, People’s Republic of China , Sun, Zhong Department of Nutrition and Food Hygiene - School of Public Health - Tianjin Medical University, Tianjin, People’s Republic of China , Huang, Guowei Department of Nutrition and Food Hygiene - School of Public Health - Tianjin Medical University, Tianjin, People’s Republic of China
Abstract :
A sensitive competitive inhibition enzyme-linked immunosorbent assay (CIELISA) for the detection of parathion has been developed. In this assay, a small molecule hapten II (O,O-diethyl O-4-aminophenyl phosphorothioate) was covalent linked to glutaric dialdehyde treated-microtiter plates. In addition, 4-(ethoxy(4-nitrophenoxy) phosphorothioylamino) butanoic acid-ovalbumin (hapten I–OVA) conjugate served as the coating antigen for comparison with directly hapten II covalent linked plates in the CIELISA format. The developed assay demonstrated highly sensitivity (IC10 was 0.08 ng mL−1) selectivity and stability. In samples analysis, the results of parathion detected by this assay were in accordance with which obtained by high-performance liquid chromatography.
Keywords :
Competive inhibition enzyme-linked immunosorbent assay , Covalent linkage , Parathion , Small molecule hapten
