Author/Authors :
Abdullahi, Masooma Diagnostic Laboratory Sciences and Technology Research Center - School of Paramedical Sciences - Shiraz University of Medical Sciences, Shiraz, Iran , Ranjbaran, Reza Shiraz University of Medical Sciences, Shiraz, Iran , Alyasin, Soheaila Department of Clinical Allergy and Immunology - Shiraz University of Medical Sciences, Shiraz, Iran , Keshavarz, Zeinab Diagnostic Laboratory Sciences and Technology Research Center - School of Paramedical Sciences - Shiraz University of Medical Sciences, Shiraz, Iran , Ramezani, Amin School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences - Shiraz, Iran , Behzad-Behbahani, Abbas Diagnostic Laboratory Sciences and Technology Research Center - School of Paramedical Sciences - Shiraz University of Medical Sciences, Shiraz, Iran , Sharifzadeh, Sedigheh Diagnostic Laboratory Sciences and Technology Research Center - School of Paramedical Sciences - Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
Background: Asthma is very common in children and its diagnosis is based on clinical
manifestations, which can be misdiagnosed as other respiratory diseases with similar
signs and symptoms. Objective: To analyze the expression of ST2L and CD203c in the
diagnosis of pediatric asthma. Methods: Basophils were purified from whole blood
samples of patients and healthy controls using Ficol-Paque gradient and Basophil
Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of
cDNA, the gene expression was analyzed by means of real time PCR. Results: Patients
expressed significantly higher levels of CD203c than healthy controls (p=0.01).
Although there was an increase in the transcription level of ST2L gene in patients, the
results were not statistically significant compared to those obtained from the healthy
controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were foundusing ROC
curve for CD203c expression. Patients with positive family history of asthma exhibited
more CD203c and ST2L expression (p<0.05). Conclusion: It is proposed that
determining CD203c expression by real time PCR may be an effective technique for
diagnosis of pediatric asthma.
Keywords :
Asthma , Basophil , ST2L , CD203c
