Temperature-assisted ionic liquid-based dispersive liquid–liquid microextraction with following back-extraction for HPLC/UV–Vis determination of 3-indole acetic acid in pea plants

In this work, the ionic liquid (IL)[C6mim][PF6] was used as IL-based extractant for dispersive liquid–liquid microextraction, followed by back-extraction and HPLC/UV–Vis determination of 3-indole acetic acid (IAA) in pea plant. The effects of some crucial factors such as chemical structure and volume of IL, pH adjustment, dissolution temperature, extraction time, centrifugation time, and ionic strength of aqueous sample were studied. The linear range of the HPLC method for IAA quantification was 17.5 × 10−2–36.8 mg L−1. LOD, LOQ, method recovery, and preconcentration factor values were 0.170 mg L−1, 0.175 mg L−1, 98.3, and 40 %, respectively. The RSD for the suggested method was calculated as 0.93 % at 35.04 mg L−1 of IAA and each IL phase was able to be reused for at least four DLLME/back-extraction cycles. To evaluate the applicability of the suggested method, IAA was determined in pea plant samples.