Author/Authors :
Khaligh, Pooneh Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran , Salehi, Peyman Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran , Moridi Farimani, Mahdi Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran , Ali-Asgari, Safa Department of Chemistry - Faculty of Science - Shahrood Branch Islamic Azad University, Shahrood , Esmaeili, Mohammad Ali Department of Biology - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University,Tehran , Nejad Ebrahimi, Samad Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran
Abstract :
Phytochemical investigation of EtOAc extract of Smilax excelsa has led to isolation and structure elucidation of five compounds. The structures of these compounds are established by different spectroscopic techniques including 1D and 2D-NMR, HRMS and ECD spectroscopy. The compounds were: solanesol (1), violasterol A (2), trans-resveratrol (3), 5-O-caffeoylshikimic acid (4) and 6-O-caffeoyl-β-d-fructofuranosyl-(2-1)-α-d-glucopyranoside (5). The configuration of compound 2 was established by electronic circular dichroism (ECD) spectroscopy. Meanwhile the cytotoxicity and antibacterial activity of the compounds were evaluated by MTT and MIC assays. Compounds 1 and 2 showed promising inhibition on MCF-7 cell line with IC50 of 161.6 and 190.0 µM, respectively. Also compounds 2 and 3 illustrated activity against Staphylococcus aureus with MIC values of 142.5 and 136.9 µM, respectively.
Keywords :
Smilax excelsa , Violasterol A , Absolute configuration , Cytotoxicity , Antibacterial
