Author/Authors :
Nazarian Hamid نويسنده , Piryaei Abbas نويسنده Shaheed Beheshti University of Medical Sciences, Piryaei A , Ardeshirylajimi Abdolreza نويسنده Stem Cell Biology Department, Stem Cell Technology Research Center , Norouzian Mohsen نويسنده Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ghoreishi Seyed Kamran نويسنده Department of Statistics, University of Qom, Qom, IR
Iran , Abdollahifar Mohammad Amin نويسنده Department of Anatomical Sciences and Biology, School of
Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
Iran , Sanchooli Tayebe نويسنده Department of Biology and Anatomical Sciences, School of
Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
Iran
Abstract :
Background Tissue engineering is an available treatment for large
bone defects. The therapeutic effects of mesenchymal stem cell (MSC) are
mostly attributed to secretion of many cytokines and growth factors.
Many factors of MSC secretions accumulate in a conditioned medium and
these factors recruit native cells into a defect site to generate new
bone tissues. Objectives The aim of this study was to evaluate the
influence of adipose tissue derived MSCs-conditioned medium (ADMSC-CM)
on bone repair of rats with critical - size calvarial defect. Methods
This experimental study was performed at Shahid Beheshti University of
Medical Sciences, Tehran, Iran, from 2016 to 2017. Conditioned medium
was collected from healthy rat adipose tissue derived MSC (ADMSC) at
passage four. Calvarial bone defect was created in hypothyroid rats
using a dental bur. Sampling was taken by the linear-mono-gram method to
determine sample size (n = 6 per group). The rats were divided randomly
into four groups based on graft material as follows: empty defect,
scaffold (Bio-Oss / type I collagen gel), scaffold / ADMSCs, scaffold
/ADMSC- CM. Evaluations were made at 4 and 8 weeks after surgery using
stereological analysis. Results Histological analysis at 8 weeks
indicated that the newly regenerated tissue almost covered the defect in
the ADMSC-CM group. Stereological analysis showed that ADMSC-CM
increased regenerated bone and numbers of osteocytes and osteoblasts
compared with the defect and scaffold groups (P < 0.05). Also,
bone regeneration was more effective in animals treated with ADMSC-CM
than in those received ADMSC. Conclusions These results suggest an
important role for ADMSC-CM in bone regeneration, through trophic impact
of its cytokines and growth factors that induce native cell
proliferation and migration into the defect. Thus, ADMSC-CM seems to
have good potential for application in bone tissue regeneration, in the
cases of hypothyroidism.
