Title of article :
Immunohistochemical Expression of P16ink4a in
Colorectal Adenocarcinoma Compared to Adenomatous and Normal Tissue Samples: A
Study on Southeast Iranian Samples
Author/Authors :
Heidari Zahra نويسنده Genetic of Non-communicable Diseases Research Center, Department of Histology, Zahedan University of Medical Sciences, Zahedan, Iran Heidari Zahra , Mahmoudzadeh Sagheb Hamidreza نويسنده Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran , Charkhat Gorgich Enam Alhagh نويسنده Student Research Committee, Zahedan University of Medical
Sciences, Zahedan, IR Iran
Abstract :
Background Colorectal cancer (CRC) is one of the most common
neoplasms of the digestive system with high morbidity and mortality.
Treatment of CRC in advanced metastatic stages is problematic. Thus,
early diagnosis in primary stages using sensitive molecular markers
seems to be necessary. Objectives This study aimed at investigating the
immunohistochemical expression of P16ink4a in CRC
compared to adenomatous and normal colorectal tissue samples of a
population from the southeast of Iran. Methods This case-control study
was conducted on 137 colorectal formalin fixed paraffin-embedded tissue
blocks for P16ink4a a protein using
Immunohistochemistry (IHC). The tissue blocks were categorized in 3
groups, including adenocarcinoma (n = 63), adenomatous (n = 38), and
normal (n = 36). All tissue blocks were collected from the pathology
department of Ali-Ebne-Abitaleb central and referral Hospital, Zahedan,
Iran from 2010 to 2015. The sections were evaluated using
semi-quantitative scoring. The P16ink4a
expression was reported as negative and positive. Clinicopathological
characteristics were also assessed. The data were analyzed by
Kruskal-Wallis and Fisher exact tests. The significance level was
considered as P < 0.05. Results The expression of
P16ink4a in adenocarcinoma, adenomatous, and
normal colorectal tissues was 25.40%, 50.00%, and 69.50%, respectively.
The P16ink4a expression was significantly higher
in non-neoplastic tissues compared to the adenomatous and colorectal
tissues (P < 0.001). There was a significant association between
P16ink4a expression and differentiation grade (P
< 0.001) and primary location of the tumor (P = 0.010).
Conclusions Considering the significant expression of
P16ink4a in normal compared to adenomatous and
cancerous samples, it seems that this biomarker could be used as a
potential useful predictor for screening and diagnosis of CRC patients
in early stages. Further researches should be conducted on this matter.
Journal title :
Astroparticle Physics
