Author/Authors :
Omrani Davood نويسنده , Kazerouni Faranak نويسنده Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University, MC, Tehran , Dehghan Nayeri Nasrin نويسنده Department of Proteomics, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran , Rahimipour Ali نويسنده Proteomics Research Center, Faculty of Paramedical Sciences , Shanaki Mehrnoosh نويسنده Department of Laboratory Medicine, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Shanaki Mehrnoosh , Rezapour Kalkhoran Maryam نويسنده Department of Medical Laboratory Sciences, School of
Allied Medical Sciences, Shahid Beheshti University of Medical
Sciences, Tehran, IR Iran , Younesian Ommolbanin نويسنده Department of Medical Laboratory Sciences, School of
Allied Medical Sciences, Shahid Beheshti University of Medical
Sciences, Tehran, IR Iran , Cheshmi Fatemeh نويسنده Department of Medical Laboratory Sciences, School of
Allied Medical Sciences, Shahid Beheshti University of Medical
Sciences, Tehran, IR Iran
Abstract :
Background Fatty acid synthase is a multifunctional protein that
catalyzes de novo synthesis of long-chain fatty acids. FASN expression
is higher in HER2-positive cells, such as SKBR3 and MCF-7/HER2 cells,
than in MCF-7 cells, which express lower HER2 levels. Curcumin, a
yellow-colored hydrophobic polyphenol derived from the rhizome turmeric,
significantly suppressed growth of human breast cancer cells. In this
study, we assessed the effect of curcumin on expression and activity of
fatty acid synthase in SK-BR-3 breast cancer cells. Objectives In this
study, we decided to determine the effects of Curcumin on Fatty Acid
Synthase expression and enzyme activity in breast cancer cell line
SKBR3. Methods We assessed the cytotoxicity effect of curcumin in
SK-BR-3 cells by MTT. Apoptosis was performed by flow cytometry. FAS
activity was measured by a spectrophotometer at 340 nm of NADPH
absorption. Fatty acid synthase gene expression was analyzed by
real-time PCR. Results Curcumin could decrease cell viability and induce
apoptosis in SK-BR-3 cells. Curcumin also reduces the enzyme activity
and expression of fatty acid synthase. Conclusions It is possible that
inhibitory effects of curcumin on FAS may induce apoptosis in SK-BR-3
breast cancer cells.
