Author/Authors :
Jabbara Neda نويسنده Department of Genetics, Faculty of Basic Sciences, Islamic
Azad University, Zanjan Branch, Zanjan, Iran , Asaadi Tehrani Golnaz نويسنده Department of Genetics, Faculty of Basic Sciences, Islamic
Azad University, Zanjan Branch, Zanjan, Iran , Lalooha Fatemeh نويسنده Department of Obstetrics and Gynecology, Qazvin University
of Medical Sciences, Qazvin, Iran , Farzam Seyed Amir نويسنده Department of Pathology, Medical Faculty, Qazvin
University of Medical Sciences, Qazvin, IR Iran , Elmizadeh Khadijeh نويسنده Department of Obstetrics and Gynecology, Qazvin University
of Medical Sciences, Qazvin, Iran
Abstract :
Introduction The endometrial cancer (EC) is the seventh most
common malignancies worldwide among females with good prognosis in early
stages of the disease. The CpG Island in the promoter region of
tumor-suppressor genes are frequently methylated in various types of
human cancers. In the present study, we investigated the methylation
pattern in promoter region of RASSF1A and RASSF2A
genes in Endometrial cancer patients in Iranian women to identify
correlations among promoter hypermethylation, disease risk and
clinicopathological parameters. Methods 28 patients and 22 healthy
controls were studied. Isolation of genomic DNA from FFPE and peripheral
blood was performed and Methylation-Specific PCR (MSP) was applied for
analysis of the promoter CpG methylation status of
RASSF1A and RASSF2A genes in the studied population.
Results A significant difference was found among the study groups and
the presence of promoter CpG hypermethylation status in the
RASSF1A (P = 0.0321) and RASSF2A
(P = 0.0003) genes. RASSF1A, and
RASSF2A gene promoter methylations were present in
53.57% and 42.85% of EC samples when compared to those in the controls
with 31.81% and 9.09% respectively. Furthermore, methylation status
between tissue and blood samples of RASSF1A, and
RASSF2A genes was not significant (P = 0.49 and 0.09 respectively). Our
results indicated a corollation between ages, menosososal state and
tumor grade with RASSF1A, and
RASSF2A promoter methylation. Conclusions In our
study, Hypermethylation of both RASSF1A and
RASSF2A genes are important events in carcinogenesis
of endometrial cancer. Epigenetic alternations may have diagnostic value
for early diagnosis and better clicinal management of susceptibility to
endometrial malignancies.
