Author/Authors :
Ghanbarzadeh Nahid نويسنده , Haghighi Fatemeh نويسنده , Mirzaei Jamal نويسنده , Khazaei Zohreh نويسنده Department of Gynecology, Birjand University of Medical Sciences, Birjand, Iran , Sharifzadeh Gholamreza نويسنده Epidemiologist, Birjand University of Medical Sciences, Birjand , Ziaee Masood نويسنده Department of Internal Medicine, Hepatitis Research Center, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, IR Iran , Behravan Mahmoodreza نويسنده Birjand Hepatitis Research Center and Department of Microbiology, Birjand University of Medical Sciences, Birjand, Iran Behravan Mahmoodreza , Javanmard Davod نويسنده Hepatitis Research Center, Birjand University of Medical Sciences, Birjand, IR Iran , Namaei Mohammad Hasan نويسنده Hepatitis Research Center, Birjand University of Medical Sciences, Birjand, IR Iran , Ghannadkafi Malaknaz نويسنده Faculty of Nursing and Midwifery,Department of Midwifery,Birjand University of Medical Sciences,Birjand,Iran , Zangoie Mahboobeh نويسنده Department of Gynecology, Birjand University of Medical
Sciences, Birjand, Iran , Naseh Narges نويسنده Department of Gynecology, Birjand University of Medical
Sciences, Birjand, Iran , Ataei Marzieh نويسنده Department of Gynecology, Birjand University of Medical
Sciences, Birjand, Iran , Salehabadi Alireza نويسنده Infectious Diseases Research Center, Birjand University of
Medical Sciences, Birjand, Iran
Abstract :
Background Infection by certain types of human papilloma virus
(HPV) is known as a causal and essential factor for cervical cancer, the
second most common malignancy in women around the world. Objectives The
aim of this study was to determine the frequency and types of HPV among
women with normal and abnormal cytology in Southern Khorasan, eastern
Iran. Methods This was a cross-sectional study with 253 randomized Pap
smear samples from women who were referred to gynecologist clinics.
Human papillomavirus-DNA testing (a nested PCR with primers MY09/ MY11
and GP5 +/ GP6 +) was performed on Pap smear samples. The first round
PCR product was subjected for sequencing to determine the HPV types.
Phylogenic analysis with Mega 6 was carried out to determine the
relationship between HPV types. Results The mean age of patients were
34.47 ± 5.38 years; 85.77% with normal cytology, and the rest were with
an abnormality; atypical cells of undetermined significance (ASCUS) and
Low-grade squamous intraepithelial lesions (LISL). Human papilloma
virus-DNA was detected in 18.57% of population (15.66% of normal and
36.11% of abnormal group) with the most prevalent HPV types 6 and 11.
The HPV type 84 was identified in a case. Conclusions The result of this
study revealed a partially high prevalence of HPV in women with normal
cytology which are high risk for transmission in population. It is
suggested that HPV testing should be carried out along with Pap test in
screening programs to enhance early detection of neoplasia and intended
infections.
