Author/Authors :
Nasr Esfahani Bahram نويسنده Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran , Halaji Mehrdad نويسنده Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran Halaji Mehrdad , Azimian Amir نويسنده Department of Pathobiology and Laboratory Science, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran , Havaei Seyed Asghar نويسنده Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , Ebrahimzadeh Namvar Amirmorteza نويسنده Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, IR Iran , Vidovic Sinisa نويسنده Vaccine and Infectious Disease Organization, University of
Saskatchewan, 120 Veterinary Road, Saskatoon, Canada , R. Dillon Jo-Anne نويسنده Vaccine and Infectious Disease Organization, University of
Saskatchewan, 120 Veterinary Road, Saskatoon, Canada , Karbalaei Mohsen نويسنده Antimicrobial Resistance Research Center, School of
Medicine, Mashhad University of Medical Sciences, Mashhad,
Iran , Ghanbari Fahimeh نويسنده Department of Microbiology, Faculty of Bioscience, Islamic
Azad University, Falavarjan Branch, Isfahan, Iran , Havaei Seyed Mehdi نويسنده Department of Biology, Faculty of Sciences, Shiraz
University, Shiraz, Iran
Abstract :
Background Staphylococcus aureus is one of the
major causes of community- and hospital-acquired infections, with
methicillin-resistant strains showing the highest rates of morbidity and
mortality. In our previous experiment, isolates, which were also used in
the present study, were assessed using multilocus sequence typing
(MLST). The sequence types (STs) were determined and documented in the
corresponding database. Objectives In the current study, the isolates
were subjected to genotyping with coagulase, SCCmec,
and agr typing methods. Methods A total of 54
isolates were evaluated by polymerase chain reaction (PCR) assay for
mecA gene, Sccmec typing, and
finally PCR-restriction fragment length polymorphism (RFLP) for
coagulase (coa) gene using Alul enzyme. MLST of the
isolates showed that the majority of methicillin-resistant S.
aureus (MRSA) isolates belonged to ST239. Results Phenotypic
and genotypic tests revealed that 21% of the isolates were MRSA.
PCR-RFLP test for coa gene showed similar patterns of
MRSA isolates. The majority of the isolates were community-acquired and
belonged to the Sccmec type IV, whereas the remaining
were hospital-acquired and classified as type I (22.2%) and type III
(2.2%). Conclusions Most of the isolates belonged to
agr type I, followed by type II and type III. Agar
dilution method showed higher sensitivity and specificity, compared to
the disk diffusion method. The majority of the isolates were
community-acquired and belonged to Sccmec type IV and
agr type I, whereas the remaining were
hospital-acquired and classified as types I (22.2%), type III (2.2%),
and agr type I.
