Author/Authors :
Bineshian Farahnaz نويسنده , Nazari Hossein نويسنده , Mehrzad Jamshid نويسنده Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur , Bakhshandeh Neda نويسنده Applied Agriculture Branch, Semnan Applied Sciences and Technology University, Semnan, Iran. Bakhshandeh Neda , Gholami Mahbubeh نويسنده Department of Biochemistry, Faculty of Medicine, Seman
University of Medical Sciences, Semnan, Iran
Abstract :
Background Nowadays, the use of herbal medicines in the prevention
and treatment of diseases has been increased in the world, especially in
Iran. Developing chemical drugs resistance in some
Candida species and their side effects, conducting
research to find new resources, especially medicinal plants, are of
prime importance. Objectives The present study aimed at evaluating
anti-Candida activities and antioxidant function of
hydroalcohlic extract of seeds of Rumex obtusifolius
and analyzing GC mass for the determined material, which included
especial formulated extract. Methods The Rumex
obtusifolius seeds were extracted using Ethyl acetate:
methanol: distilled water (6:3:1) by Soxhelet system. The antifungal
activity as well as total free phenolics and flavonoids content were
examined. The extract was carried out against 40 isolated
Candida species such as Candida
albicans and C. glabrata through well
diffusion method. In addition, hydroalcoholic extraction of R.
obtusifolius was evaluated for its antioxidant capacities
using 1-diphenyl-2-picrylhydrazyl radical scavenging. The components of
the extract were analyzed via Gas chromatography-Mass spectrometry
(GC-Mass) instrument. Results The minimum inhibitory concentration (MIC)
values were 100 - 150 μg/μL for C. albicans 150 μg/μL
for C. glabrata. The hydroalcoholic extract can
strongly scavenge DPPH radical, and its antioxidant capacities may be
correlated with the total free phenolics and total flavonoids. This
study revealed the highest antioxidant capacity in the seeds of
R. obtusifolius compared to the control groups.
Conclusions The extract contained a high amount of phenolic compounds,
and its antioxidant activity was significant. The seed of R.
obtusifolius has strong anticandidial and antioxidant
activities, which may be due to the presence of high levels of phenolic
compounds, particularly pyrogallol.
