Author/Authors :
Sabokbar Azar نويسنده Department of Microbiology, College of Basic Sciences, Karaj Branch, Islamic Azad University, Karaj, IR Iran , Sarabi Asiabar Akram نويسنده Department of Microbiology, Karaj Branch, Islamic Azad
University, Karaj, Iran , Asadzade Aghdaei Hamid نويسنده Basic and Molecular Epidemiology of Gastroenterology
Disorders Research Center, Shahid Beheshti University of Medical
Sciences, Tehran, Iran , Reza Zali Mohammad نويسنده Department of Inflammatory Bowel Disease, Research Center
for Gastroenterology and Liver Disease, Shahid Beheshti University
of Medical Sciences, Tehran, Iran , Mehdi Feizabadi Mohammad نويسنده Department of Microbiology, School of Medicine, Tehran
University of Medical Sciences, Tehran, Iran
Abstract :
Background Ulcerative colitis is a kind of inflammatory bowel
disease that is considered as immunological response to commensal
bacteria colonizing gut lumen. Adherent-invasive Escherichia
coli strains are pathogens responsible for ulcerative colitis
disease. These bacteria have special virulence factors, including type 1
fimbriae, which could be involved in inflammatory bowel disease.
Objectives The present study was conducted to determine the prevalence
of adherent-invasive E. coli with
fimH gene isolated from Iranian patients with
ulcerative colitis. Methods Sixty intestinal biopsy samples of 30
patients with ulcerative colitis and 30 individuals without inflammatory
bowel disease were examined. Biopsies from rectum, descending,
ascending, terminal ileum, and colon were taken during colonoscopy.
Results All biopsy samples were cultured for isolation of E.
coli strains. Using polymerase chain reaction assay, the
invasive plasmid antigen H and invasion-association locus genes were
detected from both isolated bacteria and tissue specimens to confirm the
presence of adherent-invasive E. coli. The frequency
of adherent-invasive E. coli with type 1 fimbriae was
much higher in patients with ulcerative colitis than control subjects.
Among isolated bacteria, type 1 fimbriae of adherent-invasive
E. coli were detected in 53.3% and 13.3% of
ulcerative colitis patients and control subjects, respectively. In
addition, from 60 biopsy samples, type 1 fimbriae were detected in 56.7%
of ulcerative colitis patients but in 10% of healthy subjects.
Conclusions Subjects without inflammatory bowel disease had a high rate
of E. coli strains than patients with ulcerative
colitis via cultivation detection. We found a high rate of type 1
fimbriae of adherent-invasive E. coli in ulcerative
colitis patients by polymerase chain reaction assay. It appears that the
presence of adherent-invasive E. coli with type 1
fimbriae in the gastrointestinal tract of patients with ulcerative
colitis is more likely than previously supposed.
