In vitroantibacterial activity of Peganum harmala(L) extract to some fish pathogenic bacteria

This study was conducted to examine in vitroantibacterial potential from seed methanol extract of Peganum harmala(L) against some fish pathogenic bacteria including Lactococcus garvieae, Aeromonas hydrophila, Yersinia ruckeriand Pseuodomonas putidaisolated from diseased rainbow trout (Oncorhynchus mykiss). The antibacterial activity of extracts was evaluated using disc diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). MICS were measured by serial dilution and the microplate assays. Results showed that the methanol extract of P. harmalawas bactericidal for all test bacteria. The MICs of extract using serial dilution and microplate method were 0.6 mg mL-1 and 0.312 to 0.625 mg mL-1 against P. putidaand 0.8 mg mL -1 and 0.625 to 1.25 mg mL -1 against L. garvieae, A. hydrophila, Y. ruckeri, respectively which was confirmed by MBC determination. Thus, the antibacterial activity of seed extract of P. harmalacan be comparable as an alternative in the control of infectious by these microorganisms