Author/Authors :
Zeinaddini Meymand Z نويسنده Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran , Yeganeh S نويسنده - Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran , GhRahimi-Mianji Gh نويسنده Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
Abstract :
The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two fragments of 171 and 362 bp from 5ʹ-UTR and 3ʹ-UTR regions of IGF-I gene were amplified, respectively. Genotyping of individuals by SSCP technique showed five banding patterns of A, B, C, D and E for 5ʹ-UTR region with the frequencies of 29.2, 0.76, 16.92, 51.53 and 10% respectively in one year-old and three banding patterns of A, C and D with the frequency of 45, 10 and 45% for two year-old fish. Also three banding patterns (A, B and C) were seen for 3ʹ-UTR region with the frequency of 62.3, 27.69 and 10.76% in one-year-old and 20, 60 and 20% in two year-old fish. The A banding pattern in 3ʹ-UTR and D banding pattern in 5ʹ-UTR sites were the most frequent pattern in the studied beluga population. The association analysis using SAS statistical software indicated no significant association between observed banding patterns and growth traits (body length, weight, and condition factor) in beluga. Considering the important role of IGF-I as a probable candidate gene affecting growth related traits, these marker sites should be studied more in larger sample sizes and also in other regions of the gene.
