Author/Authors :
Haghjooy Javanmard Shaghayegh نويسنده Physiology Research Center , Vaseghi Golnaz نويسنده Physiology Reseach Center, Isfahan University of Medical Sciences, Isfahan , Zarrin Bahareh نويسنده Applied Physiology Research Center, Cardiovascular Research Institute,Department of Physiology,Isfahan University of Medical Sciences,Isfahan,Iran , Samani Fariba نويسنده Applied Physiology Research Center, Cardiovascular Research Institute, School of Medicine,Department of Physiology,Isfahan University of Medical Sciences,Isfahan,Iran , Tajadini Mohamadhasan نويسنده Applied Physiology Research Center, Cardiovascular
Research Institute, Isfahan University of Medical Sciences, Isfahan,
IR Iran , Jamady Maryam نويسنده Applied Physiology Research Center, Cardiovascular
Research Institute, Isfahan University of Medical Sciences, Isfahan,
IR Iran , Esmaeli Neda نويسنده Applied Physiology Research Center, Cardiovascular
Research Institute, Isfahan University of Medical Sciences, Isfahan,
IR Iran , Ghasemi Ahmed نويسنده Applied Physiology Research Center, Cardiovascular
Research Institute, Isfahan University of Medical Sciences, Isfahan,
IR Iran
Abstract :
Background Metastasis is one the most important causes of death
among the patients diagnosed with breast cancer. Tau protein is a
cytoskeletal protein which competes with paclitaxel and attenuates its
effects. So increased tau protein may promote metastasis progression.
Duloxetine is an antidepressant inhibiting serotonin, norepinephrine
reuptake. In this study we tried to investigate the effects of
duloxetine in tau gene expression in breast cancer
cell line. Methods Duloxetine was prepared with the doses of 2, 20 and
100 µM per litter. MCF7 (Michigan Cancer Foundation-7) cell line was
treated for 24 hours. The toxicity of duloxetine was estimated by 3-(4,
5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT )
assay and the extent of tau protein expression was evaluated by
quantitative real-time polymerase chain reaction (qRT-PCR). Results
Statistically all concentrations of duloxetine significantly decreased
cell viability in comparison to control groups. However, paclitaxel
showed more cytotoxicity effects. According to the results of qRT-PCR,
tau expression increased at 20 and 100 µM concentration of duloxetine.
Conclusions It seems that duloxetine, a drug for pain relief in patients
with cancer, should be used with caution for metastasis promotion in
breast cancer patients.
