Author/Authors :
Fattahi moghadam Mohamad Reza نويسنده , Sarvari Jamal نويسنده Department of Bacteriology and Virology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran , Moattari Afagh نويسنده Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, IR Iran , Pirbonyeh Neda نويسنده Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, IR Iran , Hosseini Seyed Younes نويسنده Gastroenterohepatology Research Center (GEHRC), Shiraz University of Medical Sciences, Shiraz, IR Iran , Moayedi Javad نويسنده Shiraz Transplant Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran , Musavi Zahra نويسنده Faculty of Nursing and Midwifery,Islamic Azad University, Falavarjan branch,Isfahan,Iran , Mansouri Mehran نويسنده Gastroenterohepatology Research Center, Shiraz University
of Medical Sciences, Shiraz, Iran , Hashempoor Tayebeh نويسنده Shiraz HIV/AIDS Research Center, Institute of Health,
Shiraz University of Medical Sciences, Shiraz, Iran , Dowran Razieh نويسنده Department of Bacteriology and Virology, School of
Medicine, Shiraz University of Medical Sciences, Shiraz,
Iran
Abstract :
Background Spontaneous viral clearance occurs in 10% to 40% of
individuals after hepatitis C virus infection. Some polymorphisms in
IL-28B gene may influence the outcome of HCV infection. The present
study aimed at investigating the genotype and allele frequency of IL-28B
rs12979860 and rs8099917 SNPs in HCV infected patients in addition to
determining their association with disease outcome. Methods A total of
302 patients with chronic hepatic C infection and 36 individuals whose
infection was spontaneously cleared were included in this case-control
study. The presences of chronic or spontaneously cleared infection in
participants were determined by serologic and molecular methods. Genomic
DNA of the participants was extracted using salting out method.
IL-28B/IFN-λ3 gene polymorphisms were conducted using polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP). Results
The frequency of CC genotype (P = 0.001) and C allele (P = 0.0007) of
IL-28B gene at rs12979860 SNP was significantly higher in participants
with spontaneously cleared HCV infection compared to that of those who
were chronically infected. In the case of rs8099917 SNP of IL-28B, no
correlation was found between frequency of genotype (P = 0.17) or allele
(P = 0.12) and HCV infection outcome. The results of haplotype analysis
showed the association of CT (P = 0.012) and TT (P = 0.013) haplotypes
with spontaneous clearance and chronic infection, respectively.
Conclusions The findings implied that individuals with CC or CT genotype
at rs12979860 SNP but rs8099917 SNP was not in association with
spontaneous clearance of HCV in an Iranian population with HCV
infection.
