Author/Authors :
Mohammadi Mohsen نويسنده , Karami Hadi نويسنده Department of Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran Karami Hadi , Farajollahi Alireza نويسنده Department of Medical Physics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Nejati-Koshki Kazem نويسنده Tabriz Genetic Analysis Center(TGAC) , Tabriz University of Medical Sciences, Tabriz, Iran , Pirayesh Islamian Jalil نويسنده Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Oladghaffari Maryam نويسنده Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract :
[Background]Radio therapy plays an important role in controlling tumor growth in esophageal cancer patients.[Objectives]Our study provided comprehensive information about radio-sensitivity of Hdm2 gene in esophageal cancer cells in cancer cells.[Methods]The expression of Hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma TE1, TE8 and TE11 cell lines is studied. RNA extracted from each esophageal cancer cell, then Quantitative reverse transcription PCR (RT-qPCR) assay were used for measuring HDM2 gene expression. Finally, radiosensitivity was determined for every three esophageal cancer cell lines.[Results]It was found that Hdm2 gene significantly expressed in TE1, TE8 and TE11 cell lines by qRT-PCR analysis. Clonogenic assay showed that TE-11 was very sensitive to radiation relative to other cell lines.[Conclusions]Hdm2 protein binding to the P53 protein is a key regulator of cell cycle control and genome protection Cell cycle control, programmed cell death and response cells to radition play important roles.
