Author/Authors :
Ataee Ramezan Ali نويسنده Department of Medical Microbiology, Therapeutic Microbial Toxin Research Center, Faculty of Medicine, Baqiyatallah University of Medical Sciences, , Rashki Mahdi نويسنده Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran , Alishiri Gholam Hossien نويسنده Department of Rheumatology, Faculty of Medicine, and Hospital Research Development Committee, Baqiyatallah University of Medical Sciences, Tehran, IR Iran , Esmaeili Davoode نويسنده Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract :
[Background]The role of Staphylococcal enterotoxin E (Superantigen E) in rheumatoid arthritis pathogenesis has been considered. This paper aimed at determining Staphylococcal enterotoxin E in the synovial fluid of rheumatoid arthritis patients.[Methods]In this study, 100 synovial fluid samples of patients with rheumatoid arthritis were examined. The primers pairs were designed based on the S. baureus enterotoxin type E (entE) gene, GenBank: M21319.1. All samples were subjected to DNA extraction separately. Then, polymerase chain reaction (PCR) was implemented. Data were analyzed by descriptive statistics.[Results]The PCR results indicated that Staphylococcal enterotoxin E gene existed in synovial fluid samples of 25% of patients with rheumatoid arthritis, with a high percentage.[Conclusions]The study results revealed that a high percentage of patients with rheumatoid arthritis have Staphylococcal enterotoxin type E gene in their synovial fluid. However, further studies are needed to assess other Staphylococcal enterotoxins. This finding may provide a model for diagnosing rheumatoid arthritis disease. The results of this study have presented some evidence regarding endogenous origin of involved superantigen in patients with rheumatoid arthritis.
