Title of article :
The Effect of Ethanol Baneh Skin Extract on the Expressions of Bcl - 2, Bax, and Caspase - 3 Concentration in Human Prostate Cancer pc3 Cells
Author/Authors :
Namaki Saeed نويسنده , Kazerouni Faranak نويسنده Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University, MC, Tehran , Amiri Maryam نويسنده , Rahimipour Ali نويسنده Proteomics Research Center, Faculty of Paramedical Sciences , Ebrahimi Nazanin نويسنده Departments of Biochemistry, Medical School, Kerman University of Medical Sciences, Kerman, IR Iran , Barghi Siamak نويسنده 5Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Nasrollahi Foad نويسنده Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rajizadeh Afsaneh نويسنده MSc of Nutrition in Public Health, Health Services Management Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran , Dehghan Nayyeri Nasrin نويسنده Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ahmadi Hadis نويسنده Department of Biochemistry, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Abstract :
[Background]Traditional herbs are effective in the treatment of many diseases. It is proven that traditional herbs can decrease the risk of cancer and grow the survivals of patients.[Objectives]Based on the results of our previous research, ethanol Baneh skin extract has cytotoxic effect on pc3 cells; thus, we decide to determine the effect of Baneh extracts on Bcl2 and Bax expression and amount of concentration of caspase3.[Methods]The expressions of apoptotic - related genes were determined by real time PCR and the concentration of caspase3 was determined by ELISA method.[Results]Ethanol Baneh skin extract increased the mRNA expression of Bax and decreased the mRNA expression of Bcl2 in pc3 cells and also increased the amount of caspase3.[Conclusions]The result of this study indicates the Ethanol Baneh skin extract including photochemical that might be a candidate for the herbal anti - cancer drugs.
