Author/Authors :
Shaiegan Mojgan نويسنده , Mohammadi Saeed نويسنده Faculty of Advanced Medical Technology, Golestan University of Medical Sciences, Gorgan, IR Iran Mohammadi Saeed , Rahmani Babak نويسنده Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Rahmani Babak , Nikbakht Mohsen نويسنده Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Nikbakht Mohsen , Zahedpanah Mahdi نويسنده Blood Transfusion Research center,High Institute for Research and Education in Transfusion Medicine,Tehran,Iran , Nikugoftar Mahin نويسنده Blood Transfusion Research center,High Institute for Research and Education in Transfusion Medicine,Tehran,Iran , Ghaffari Seyed Hamidollah نويسنده Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran , Hamedi Asl Dariush نويسنده Department of Biochemistry and Genetics, Qazvin University of Medical Sciences, Qazvin, Iran
Abstract :
[Background]Daunorubicin (DNR) is capable of killing the human acute myeloid leukemia cells through apoptosis or necrosis with arresting cell cycle and various mechanisms. The response of AML cells to DNR associated with defect and or defiance in survival has been a consideration subject.[Objectives]We have represented the transcription gene profile of some critical prosurvival proteins by qRT - PCR, including osteopontin (OPN), AKT1, mTOR, β - catenin, and NF - kB/RelB.[Methods]The U937 cells were treated with DNR with clinically achievable concentrations for MTT assay, annexin V (AV) /Propidium iodide (PI), and cell cycle analysis. QRT - PCR was performed, using primers of OPN, NF - kB/RelB, AKT1, mTOR, PTEN, and β - catenin.[Results]The AV/PI assay displayed that DNR - induced death in cells was a dose - dependent and necrotic manner. Cell cycle distribution following treatment with DNR exhibited a relatively lower chromatin of S phase than untreated cells. OPN gene expression was significantly attenuated. NF - kB/RelB, mTOR, β - catenin, as well as PTENgenes showed unchanged or non - significant increase in expression. However, AKT1increased significantly.[Conclusions]U937 sensitivity to DNR could be due to the targeting of anti-apoptotic proteins in the transcriptional stages. The decrease in OPNlevels appears to play a significant role in the death of the observed by DNR.
