Author/Authors :
Abedi Parvin نويسنده Faculty of Nursing and Midwifery, Ahvaz Medical University of Jondi Shapur, Ahvaz, Iran , Fatahinia Mahnaz نويسنده Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , Namjoyan Foroogh نويسنده Medicinal Plant Research Center, Department of Pharmacognosy, School of pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz , Yaralizadeh Masumeh نويسنده Midwifery Department, Menopause Andropause Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Nezamivand-Chegini Salimeh نويسنده Midwifery, Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran , Yaralizadeh Maryam نويسنده Rehabilitation Department Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract :
[Background]Black walnut (Juglans nigra) is known to have antimicrobial and antifungal effects in vitro.[Objectives]This study aimed to compare the effects of extracts derived from green-husked walnut with clotrimazole against Candida albicans in female rats.[Methods]In this study, 35 female Wistar rats were randomly assigned to 5 groups. A total of 4 groups of rats were infected vaginally with C. albicans and 1 group was not infected (negative control). The 4 infected groups received the following treatments: 2 groups received vaginal creams containing 2% or 4% of J. nigra extracts, respectively, 1 group received 1% clotrimazole, and 1 group did not receive any treatment (positive control). All rats received treatments for 2 weeks.[Results]The mean number of colony forming unit (CFUs) before intervention was 308.2 ± 8.73 and 219 ± 13.4 in the 2% and 4% J. nigra group, 312.7 ± 28.32 in the clotrimazole group, 233.85 ± 8.15 in the positive control, and 0 in the negative control group (P < 0.001). After 1 week, the number of CFUs significantly decreased to 71.1 ± 3.9 in the 2% J. nigra, 0 in both 4% J. nigra and clotrimazole groups, 230.7 ± 9.86 in the positive control, and 0 in the negative control group (P < 0.001). After 2 weeks, no CFUs were detected in any of the infected groups except for the positive control group.[Conclusions]Vaginal creams containing 4% J. nigra significantly eliminated C. albicans in female rats after 1 week and its effect was similar to that of clotrimazole.
