Author/Authors :
Jaradat Nidal نويسنده Department of Pharmacy, An-Najah National University, Palestine , Shawarb Nuha نويسنده Department of Chemistry, An-Najah National University, Palestine , Hussein Fatima نويسنده Department of Pharmacy, An-Najah National University, Palestine , Al-Masri Motasem نويسنده Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Palestine , Warad Ismail نويسنده Department of Chemistry, An-Najah National University, Palestine , Khasati Ahmad نويسنده Department of Pharmacy, An-Najah National University, Palestine , Shehadeh Mayadah نويسنده Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan,Queen Rania Street, Amman , Qneibi Mohammad نويسنده Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine , Mahmoud Ali Hussein Azmi نويسنده Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine , Makhamreh Sabha نويسنده Al-Deyaa Medical Laboratory, Hebron, Palestine
Abstract :
[Background]The semi-synthesis of drugs from natural products is still limited and complicated. Recently, there has been compelling global need to develop novel and potential antibacterial and antioxidant agents.[Objectives]The current study aimed at semi-synthesizing new derivatives from naringin, to verify their chemical structures and assess their antibacterial and antioxidant potentials.[Methods]The semi-synthesis of naringin was conducted in the presence of hydrazone and oxime derivatives in acidic solution, while elemental and spectral analytical methods were used to verify the chemical structures of the semi-synthesized molecules. Also, to assess their antibacterial activity, the micro-broth dilution method with different American type culture collection (ATCC) strains as Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and clinical isolate methicillin-resistant Staphylococcus aureus (MRSA) were utilized. Moreover, 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) was used to assess the antioxidant activity of the derived compounds.[Results]Three new hydrazone and oxime compounds were semi-synthesized from naringin and their chemical structures were identified by 13C NMR, IR, MS, and 1H NMR. Among the semi-synthesized compounds, the (2a) molecule showed the best antibacterial activity with a minimum inhibitory concentration (MIC) value of 62.5 μg/mL. Also, this compound exhibited the best antioxidant activity with IC50 3.7 μg/mL, in comparison with the other studied samples.[Conclusions]Hydrazone (2a) compound could be used as a potent antioxidant and bacterial agent. Further studies are required to investigate other therapeutic effects of the (2a) molecule.
