Author/Authors :
Moslemi Elham نويسنده Department of Microbiology, Shargh Branch, Islamic Azad University, Tehran, IR Iran , Taghavi Afsoon نويسنده Department of Medical Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , Kheiri-Manjili Hamidreza نويسنده Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran , Soltandalal Mohammad Mehdi نويسنده Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran , Beheshtizadeh Mohammad Reza نويسنده Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran , Mahmoudi Lamouki Reza نويسنده Department of Medical Genetic, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Izadi Amir نويسنده Banej Exir Research Center, Tehran, Iran
Abstract :
[Background and Objectives]Brucella is an intracellular gram-negative bacterium that can infect many kinds of mammals like humans, sheep, cattle, etc. Brucellosis is a contagious occupational disease caused by Brucella spp. that affects individuals who have close contact with infected animals. The clinical features of Brucellosis are not disease-specific and almost every organ can be affected. This zoonotic disease is a great health concern and economically important in many countries, such as Iran. The aim of this study was to detect Brucella spp. in pasteurized and non-pasteurized dairy products.[Methods]In this study, 208 samples, including goat, sheep, and cow raw and pasteurized milk as well as pasteurized and non-pasteurized cheese, were collected in Tehran province. The DNA was extracted, and then the real-time PCR was used for detection of the Brucella spp. gene.[Results]The prevalence of Brucella spp. contamination in the dairy products was: 45.5% in goat`s raw milk, 39.1% in non-pasteurized cheese, 27.3% in sheep`s raw milk, 26.3% in cow`s raw milk, 25% in pasteurized cheese, and 14.7% in pasteurized milk.[Conclusions]Rapid and exact detection of pathogens in dairy products is the most significant factor to prevent foodborne diseases. In addition, the real-time PCR assay is sensitive and specific enough to detect a low number of Brucella spp. in dairy products.
