Author/Authors :
Hojabri Zoya نويسنده Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran. , Rahmati Marveh نويسنده Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran. , Moosavi Mohammad Amin نويسنده Department of Zoology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. , Hasani Akbar نويسنده Drug Applied Research Center and Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. , Nourashrafeddin mehdi نويسنده Magee-Women Research Institute & Foundation - School of Medicine - University of Pittsburgh, Pittsburgh , Zarghami Nosratollah نويسنده Hematology and Oncology Research Center - Tabriz University of Medical Science
Abstract :
Nucleostemin (NS), a stem cell-abundant nucleolar protein, is critical for maintaining the self-renewal and proliferative properties of normal and cancerous stem cells. Recent data suggests that NS signaling is important for proliferation of T-cells and leukemia cells. This study was conducted to verify the role of NS in pathogenesis and treatment of T-cell acute lymphocytic leukemia (T-ALL). Our results revealed that RNA interference-mediated NS silencing primarily affected clonogenicproperty of T-ALL cells by limiting their self-renewal potential in vitro.These effects were accompanied with inhibition of proliferation and early apoptosis in Jurkat cells (p53-null) while late apoptosis in Molt-4 (p53 functional) T-ALL cells. Collectively, our results suggest that NS is a critical regulator in self-renewal and apoptosis of differentT-ALL cells. This suggests therapeutic potential of this gene in leukemia.
