Author/Authors :
Azad Mehdi نويسنده , Eskandari Fatemeh نويسنده Prevention of metabolic diseases research center, Research Institute of endocrine sciences , Goudarzi Mehdi نويسنده Department of Pharmacology and Toxicology, Pharmacy School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran , Allahverdi Amir نويسنده Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , Abroun Saied نويسنده Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , Moghadasi MohammadHossein نويسنده Department of Hematology - Faculty of Medicine Sciences - Tarbiat Modares University , Soleimani Masoud نويسنده Department of Hematology - Faculty of Medicine Sciences - Tarbiat Modares University
Abstract :
Lentiviral vectors (LVs) are useful vehicle for genetransfer to dividing and non-dividing cells and genetic manipulations. However, the use of lentiviruses in studies requires an accurate titration technique.Quantitative real-time PCR (qPCR) is a sensitive technique for the indication and quantitation of retrovirals particles. In this study, we used the qPCR for lentiviral vector titeration. The puromycin resistance gene as templates for an SYBR green-based real-time qPCR method and detect lentiviral copy number integrated lentiviral DNA. Consequently, this studyshowed that theusing ofantibioticresistance genesviral particles titration maybeefficient with highly accuracy.
