Author/Authors :
Nikravesh Abbas نويسنده , Khoshbaten Manouchehr نويسنده Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz , Miri Hamidreza نويسنده Department of Biology, Faculty of Basic Science, University of Zabol, Zabol, IR Iran , Bonyadi Morteza نويسنده , Abdolmohammadi Reza نويسنده Department of Animal Biology, Faculty of Natural Sciences,
University of Tabriz, Tabriz, Iran , Somi Mohammadhossein نويسنده Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran , Rahbar Kafshboran Haniyeh نويسنده Department of Biology - Faculty of Sciences - University of Zabol , Haghi Mehdi نويسنده Department of Biology - Faculty of Natural Sciences - University of Tabriz
Abstract :
BACKGROUND Inflmmatory bowel disease (IBD) is a chronic, relapsing, inflmmatory disorder of the gastrointestinal tract that includes two entities, Crohn’s disease (CD) and ulcerative colitis (UC). As with other complex diseases, both genetic susceptibility and environmental factors play role in the pathogenesis of these diseases. The tumor necrosis factor (alpha) (TNF-(alpha)) gene is located in the IBD3 region on chromosome 6p21 which is a good functional candidate for involvement in susceptibility to IBD. In addition, the promoter region of TNF-? contains various polymorphisms that have shown a signifiant association with IBD. METHODS In this case control study we investigated the TNF-(alpha) -857 polymorphism in 109 patients (89 UC and 16 CD) who suffered from IBD and 100 healthy age, sex and ethnicity matched adults selected from the same population, as the control group. The polymorphism was checked by amplifiation refractory system (ARMS) and polymerase chain reaction (PCR). RESULTS Investigation of the association of TNF-(alpha) -857 gene promoter polymorphism with both types of IBD showed no signifiant difference in genotype and allele frequencies of this polymorphism between UC patients and controls. However, a possible association of TNF-(alpha) -857 polymorphism (p=0.03) was identifid with CD. CONCLUSION TNF-(alpha) -857 polymorphism may have a role in the development of CD in the Iranian Azeri Turkish population.
