Author/Authors :
Ayatollahi, Abdul Majid School of Pharmacy and Phytochemistry Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Ghanadian, Mustafa Pharmaceutical Sciences Research Center - Isfahan University of Medical Sciences, I.R , Afsharypuor, Suleiman Department of Pharmacognosy - Faculty of Pharmacy and Pharmaceutical Sciences - Isfahan University of Medical Sciences , Mesaik, M. Ahmad Dr. Panjwani Center for Molecular Medicine and Drug Research - International Center for Chemical and Biological Sciences - University of Karachi - Karachi-75270, Pakistan , Abdalla, Omer Mohamed Dr. Panjwani Center for Molecular Medicine and Drug Research - International Center for Chemical and Biological Sciences - University of Karachi - Karachi-75270, Pakistan , Shahlaei, Mohsen Department of Medicinal Chemistry - Faculty of Pharmacy - Kermanshah University of Medical Sciences , Farzandi, Gholamhossein Welsh School of Pharmacy Centre for Socioeconomic Research - Cardiff University, UK. , Mostafavi, Hamid Welsh School of Pharmacy Centre for Socioeconomic Research - Cardiff University, UK
Abstract :
The cytotoxic chloroform fraction of Euphorbia aellenii afforded two cycloartane type triterpenes-cycloart-25-en-3β,24-diol (1) and 24-methylene-cycloartan-3β-ol (2)-for the first time from this plant. Preparation of cycloartane derivatives, 3β, 24-O-diacetyl-cycloart-25-en as compound 3 and 3β-O-acetyl-24-methylene-cycloartan (4) were conducted by acetylating of 1 and 2, respectively. The structures of the isolated compounds were elucidated by spectroscopic methods and their activities evaluated by proliferation assay on human peripheral blood lymphocytes (PBLs). Comparing the results suggested that anti-proliferation effect of these compounds on PBLs might be due to the presence of free 3-OH group while masking the free OH groups by acetylation, could induce proliferation activity.
Keywords :
Euphorbia aellenii , Proliferation assay , Cycloartanes , Structure-activity relationship , SAR , Protein kinase C , PKC
