Title of article :
Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line
Author/Authors :
Ahmadian, Nasser Faculty of Advanced Medical Science Technology - Golestan University of Medical Sciences , Pashaei-Asl, Roghiyeh Faculty of Advanced Biomedical Sciences - Tabriz University of Medical Sciences , Samadi, Nasser Faculty of Advanced Biomedical Sciences - Tabriz University of Medical Sciences, , Rahmati-yamchi, Mohammad Department of Biochemistry - Tabriz University of Medical Sciences , Rashidi, Mohammad-Reza Research Center for Pharmaceutical Nanotechnology - Faculty of Pharmacy - Tabriz University of Medical Sciences , Ahmadian, Masomeh Faculty of Advanced Medical Science Technology - Golestan University of Medical Sciences , Esmaeili, Moosa Women’s Reproductive Health Research Center - Alzahra Hospita - Tabriz University of Medical Sciences , Salamat, Faezeh Golestan Research Center of Gastroenterology and Hepatology - Golestan University of Medical Sciences , Besharat, Sima Golestan Research Center of Gastroenterology and Hepatology - Golestan University of Medical Sciences , Joshaghani, Hamid Reza Golestan Research Center of Gastroenterology and Hepatology - Golestan University of Medical Sciences
Abstract :
BACKGROUND
Hesa-A is a natural compound with anticancer properties. The exact mechanism
of its action in esophageal cancer is not clear, yet. The aim of this study was
to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell
lines, KYSE-30, and cell cycle genes expression.
METHODS
In this study, we tested cell toxicity with MTT (3-(4,5-Dimethylthiazol-
2-yl)-2,5-Diphenyltetrazolium Bromide) assay and flow cytometry to evaluate
the cell cycle arrest. Real time polymerase chain reaction was used to assess the
expression of P53, P16, P21, cyclin D1, and cyclin B1 genes.
RESULTS
Our results showed that Hesa-A is effective in the expression of cell cycling
check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell
cycle. The levels of P53 (>13 times), P21 (>21 times), P16, cyclin B1, and
cyclin D1 genes were increased 48 hours after Hesa-A treatment.
CONCLUSION
P21 and P16 expression were the potential mechanisms for G2 arrest of
KYSE-30 esophageal cancer cell line by Hesa-A.
Keywords :
Cyclin B1 gene , Cyclin D1 gene , P21 gene , P16 gene , P53 gene , Real Time PCR , Flow cytometry , Esophageal cancer , Hesa-A
Journal title :
Astroparticle Physics
