Author/Authors :
Ghanadian, Mustafa Isfahan Pharmaceutical Sciences Research Center- Isfahan University of Medical Sciences , Ayatollahi, Abdul Majid School of Pharmacy and Phytochemistry Research Centre - Shahid Beheshti University of Medical Sciences, Tehran , Afsharypour, Suleiman School of Health - Faculty of Pharmacy and Pharmaceutical Sciences - Isfahan University of Medical Sciences, Isfahan , Hareem, Sumaira Dr. Panjwani Center for Molecular Medicine and Drug Research - Sciences - University of Karachi - Karachi-75270, Pakistan , Abdalla, Omer Mohamed Dr. Panjwani Center for Molecular Medicine and Drug Research - Sciences - University of Karachi - Karachi-75270, Pakistan , Kezetas Bankeu, Jean Jules Department of Organic Chemistry - Faculty of Science - TWAS Research Unit of University of Yaounde I; Yaounde, Cameroon
Abstract :
Four known flavonoids: quercetin 3-O-β-D-rutinoside (Q3Rut), myricetin 3-O-β-D-galactopyranoside (M3Gal), quercetin 3-O-β-D-galactopyranoside (Q3Gal) and quercetin 3-O-β-D-glucopyranoside (Q3Glc), for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques. The immunomodulatory activities of isolated compounds were compared using standard T-cell proliferation assay. These data showed that lymphocyte suppression activity of flavonoids (1-4) were comparatively lower than prednisolon as a standard drug. Immunosuppressive activity of flavonoids with hydroxyl groups at both 3′-and 4′-positions in their B-ring (Q3Gal) were more than those with 3′-,4′-and 5′-hydroxyl substitution (M3Gal). In these compounds, Q3Gal showed the most inhibitory activity, whereas M3Gal showed the least lymphocyte antiprolifeartive activity.
Keywords :
Euphorbia microsciadia , Flavonoids , Immunomodulatory activity , Flavonol glycosides
