Author/Authors :
Mehdizadeh Kashani, Toktam Department of Toxicology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Vatanpour, Hossein Department of Toxicology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran - Pharmaceutical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Zolfagharian, Hossein Department of Venomous Animals and Antivenom Production - Razi Vaccine and Serum Research Institute, Karaj , Hooshdar Tehrani, Hasan Department of Medicinal Chemistry - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Heydari, Mohammad Hossein Proteomics Research Center - Paramedical School - Shahid Beheshti University of Medical Sciences, Tehran , Kobarfard, Farzad Phytochemistry Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Department of Medicinal Chemistry - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 μM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity.
Keywords :
Anticoagulant , Antiplatelet aggregation , Snake venom , Echis carinatus , Cerastes persicus fieldi , Gel filtration
