Author/Authors :
Ayatollahi, Abdulmajid Department of pharmacognosy - School of Pharmacy - Shahid Beheshti University - M.C., Tehran - School of Pharmacy and Phytochemistry Research Centre - Shahid Beheshti University of Medical Sciences, Tehran , Ajami, Marjan Faculty of Nutrition Sciences and Food Technology - Shahid Beheshti University of Medical Sciences and Health Services , Reyhanfard, Hamed School of Pharmacy and Phytochemistry Research Centre - Shahid Beheshti University of Medical Sciences, Tehran , Asadi, Yasin Physiology Research Center - Tehran University of Medical Sciences, Tehran , Nassiri-Kashani, Mansour Center for Research and Training in Skin Diseases and Leprosy - Tehran University of Medical Sciences, Tehran , Rashighi Firoozabadi, Mehdi Center for Research and Training in Skin Diseases and Leprosy - Tehran University of Medical Sciences, Tehran , Davoodi, Hossein Faculty of Nutrition Sciences and Food Technology - Shahid Beheshti University of Medical Sciences and Health Services , Habibi, Esmaeil Faculty of Library and Information Science - Islamic Azad University - Tehran Science and Research Unit, Tehran , Pazoki-Toroudi, Hamidreza Nano Vichar Pharmaceutical Ltd, Tehran - Physiology Research Center - Tehran University of Medical Sciences, Tehran - Center for Research and Training in Skin Diseases and Leprosy - Tehran University of Medical Sciences
Abstract :
Despite all modern surgical techniques, skin flap that is considered as the main method in most reconstructive surgeries puts the skin tissue at danger of necrosis and apoptosis derived from ischemia. Therefore, finding a treatment for decreasing the apoptosis derived from flap ischemia will be useful in clinic. In present study, we evaluated the effect of azelaic acid 20% and finasteride on expression of BCL-2 and bax proteins after the skin flap surgery. For this purpose, 21 rats were entered
in three groups including control, azelaic acid 20% and finasteride, all experienced skin flap surgery and then flap tissue was assessed for determining the expression of proteins in 5 slices prepared from each rat that were graded between – to +++ scales. Both azelaic acid and finasteride increased the expression of BCL-2 protein (p < 0.05) and decrease the expression of bax protein (p < 0.05). These results suggested an antiapoptotic role for finasteride and azelaic acid in preserving
the flap after the ischemia reperfusion insult.
Keywords :
Azelaic acid , Apoptosis , Finasteride , Skin flaps
