Author/Authors :
Amirahmadi, Maryam Department of Pharmacology and Toxicology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran - Food and Drug Laboratories Research Center - Ministry of Health and Medical Education, Tehran - Food and Drug Control Laboratories - Food and Drug Deputy - Ministry of Health and Medical Education, Tehran , Yazdanpanah, Hassan Department of Pharmacology and Toxicology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran - Food and Drug Laboratories Research Center - Ministry of Health and Medical Education, Tehran , Shoeibi, Shahram Food and Drug Laboratories Research Center - Ministry of Health and Medical Education, Tehran - Food and Drug Control Laboratories - Food and Drug Deputy - Ministry of Health and Medical Education, Tehran , Pirali-Hamedani, Morteza Food and Drug Laboratories Research Center - Ministry of Health and Medical Education, Tehran - Food and Drug Control Laboratories - Food and Drug Deputy - Ministry of Health and Medical Education, Tehran - Department of Pharmacology - School of Pharmacy - Tehran University of Medical Sciences, Tehran , Ostad Gholami, Mahsa Food and Drug Laboratories Research Center - Ministry of Health and Medical Education, Tehran - Food and Drug Control Laboratories - Food and Drug Deputy - Ministry of Health and Medical Education, Tehran , Farshid Mohseninia, Mohammad Food and Drug Laboratories Research Center - Ministry of Health and Medical Education, Tehran - Food and Drug Control Laboratories - Food and Drug Deputy - Ministry of Health and Medical Education, Tehran , Kobarfard, Farzad Department of Medicinal Chemistry - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran - Phytochemistry Research Center - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
A reliable, rapid and accurate method based on spiked calibration curves and direct
sample introduction was developed for determination of 17 pesticide residues in rice by gas chromatography-mass spectrometry single quadrupole selected ion monitoring GC/MS-SQ-SIM. Sample preparation is based on extraction with acetonitrile without clean up. The use of spiked calibration curves for constructing the calibration curve substantially reduced adverse matrix-related effects. The average recovery of pesticides at 6 concentration levels was in range of 97.5-102.1%. The method was proved to be repeatable with RSDr in range of 0.7%-19.8%for all of the concentration levels. The limits of detection and limit of quantifications for all the pesticides were < 10 ng/g and < 25 ng/g, respectively. The developed method was applied for simultaneous determination of the selected pesticides in 23 rice samples collected from Tehran retail market in March 2009. Although many studies have been conducted regarding the determination of pesticides by using GC-MS, this is the first attempt in Iran using GC-MS-SIM technique that successfully can determine 17 pesticides with difference in physicochemical properties in rice.
Keywords :
Pesticides , Spiked calibration curve , GC/MS , Rice
