Title of article :
In-vitro Activity of 10 Antifungal Agents against 320 Dermatophyte Strains Using Microdilution Method in Tehran
Author/Authors :
Adimi, Parvaneh Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences , Hashemi, Jamal Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences , Mahmoudi, Mahmood Department of Epidemiology and Biostatistics - School of Public Health - Tehran University of Medical Sciences , Mirhendi, Hossein Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences , Shidfar, Mohammad Reza Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences , Emmami, Masood Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences , Rezaei-Matehkolaei, Ali Department of Medical Mycology - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Gramishoar, Mohsen Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences , Kordbacheh, Parivash Department of Medical Parasitology and Mycology - Tehran University of Medical Sciences
Abstract :
Dermatophyte fungi are the etiologic agents of skin infections commonly referred to as
ringworm. These infections are not dangerous but as a chronic cutaneous infections they may be difficult to treat and can also cause physical discomfort for patients. They are considered important as a public health problem as well. No information is available regarding the efficacy of antifungal agents against dermatophytes in Tehran. Therefore, in this study we evaluated the efficacy of 10 systemic and topical antifungal medications using CLSI broth microdilution method (M38-A). The antifungal agents used included griseofulvin, terbinafine, itraconazole, ketoconazole, fluconazole, voriconazole, clotrimazole, ciclopirox olamine, amorolfine and naftifine.Fifteen different species of dermatophytes which were mostly clinical isolates were used as follows; T. mentagrophytes, T. rubrum, E. floccosum, M. canis, T. verrucosum, T. tonsurans, M. gypseum, T. violaceum, M. ferruginum, M. fulvum, T. schoenleinii, M. racemosum,
T. erinacei, T. eriotrephon and Arthroderma benhamiae. The mean number of fungi particles (conidia) inoculated was 1.25 ×10⁴ CFU/mL. Results were read after 7 days of incubation at 28 °C. According to the obtained results,itraconazole and terbinafine showed the lowest and fluconazole had the greatest MIC values for the most fungi tested. Based on the results, it is necessary to do more research and design a reliable standard method for determination of antifungal susceptibility to choose proper antibiotics with fewer side effects and decrease antifungal resistance and risk of treatment failure.
Keywords :
In-vitro Activity , Antifungal Agents against , Dermatophyte Strains , Microdilution Method , Tehran
Journal title :
Astroparticle Physics
