Title of article :
Development of a Simple RP-HPLC-UV Method for Determination of Azithromycin in Bulk and Pharmaceutical Dosage forms as an Alternative to the USP Method
Author/Authors :
Ghari, Tayebeh Department of Pharmaceutics - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran - Department of Pharmaceutical Chemistry - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Kobarfard, Farzad Department of Pharmaceutical Chemistry - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Mortazavi, Alireza Students Research Committee - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
The present study was designed to develop a simple, validated liquid chromatographic
method for the analysis of azithromycin in bulk and pharmaceutical dosage forms using ultraviolet detector. The best stationary phase was determined as C18 column, 5 μm, 250 mm × 4.6 mm. Mobile phase was optimized to obtain a fast and selective separation of the drug. Flow rate was 1.5 mL/min, Wavelength was set at 210 nm and the volume of each injection was 500 μL. An isocratic methanol/buffer mobile phase at the ratio of 90:10 v/v gave the best separation and resolution. The proposed method was accurate, precise, sensitive, and linear over a wide range of concentration of azithromycin. The developed method has the advantage of using UV detector compared to the USP method in which electrochemical detector has been used. The
validated method was successfully applied to the determination of azithromycin in bulk and pharmaceutical dosage forms.
Keywords :
Azithromycin , HPLC , UV detection , Determination , Pharmaceuticals
Journal title :
Astroparticle Physics
