Title of article :
Improvement of Capillary Electrophoretic Enantioseparation of Fluoxetine by a Cationic Additive
Author/Authors :
Sattary Javid, Farin Pharmaceutical Chemistry Department - School of Pharmacy -Shahid Beheshti University of Medical Sciences, Tehran - Students Research Committee - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Shafaati, Alireza Pharmaceutical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Pharmaceutical Chemistry Department - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Zarghi, Afshin Pharmaceutical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Pharmaceutical Chemistry Department - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
One of the problems encountered in CE separations of basic compounds is the adsorption of analytes in negatively charged capillary wall which could lead to poor repeatability of migration time and peak area. Additionally, separation of enantiomers of chiral of basic drugs is commonly carried out in low pH buffer which contributes to strong ionic interaction of the cationic drug ions with negatively charged chiral selectors. The two phenomena results in poor enantioseparations. To overcome the problems associated with chiral separations of basic drugs by CE, the effect of guanidine (GU) on the improvement of chiral separation of a model basic drug, fluoxetine (FLX), was investigated. In the present study, GU was used as a cationic
additive to the running buffer containing a chiral selector, sulfated beta cyclodextrine. Better results obtained with GU as the buffer additive in enantioseparation of FLX.
Keywords :
Capillary electrophoresis , Chiral separation , Fluoxetine , Guanidine
Journal title :
Astroparticle Physics