Author/Authors :
Amar, Yacine Laboratory of Bioconversion - Engineering and Microbiological Food Safety - University of Mascara (29000), Algeria , Meddah, Boumediene Laboratory of Bioconversion - Engineering and Microbiological Food Safety - University of Mascara (29000), Algeria , Bonacorsi, Irene Laboratory of Immunology and Biotherapy - Department of Human Pathology University of Messina, 98125- Messina, Italy , Costa, Gregorio Laboratory of Immunology and Biotherapy - Department of Human Pathology University of Messina, 98125- Messina, Italy , Pezzino, Gaetana Laboratory of Immunology and Biotherapy - Department of Human Pathology University of Messina, 98125- Messina, Italy , Saija, Antonina Department of Pharmaco-chemistry - University of Messina - Viale Annunziata, 98168 - Messina, Italy , Cristani, Mariateresa Department of Pharmaco-chemistry - University of Messina - Viale Annunziata, 98168 - Messina, Italy , Boussahel, Soulef Department of Pharmaco-chemistry - University of Messina - Viale Annunziata, 98168 - Messina, Italy , Ferlazzo, Guido Laboratory of Immunology and Biotherapy - Department of Human Pathology University of Messina, 98125- Messina, Italy , Tirtouil Meddah, Aicha Laboratory of Bioconversion - Engineering and Microbiological Food Safety - University of Mascara (29000), Algeria
Abstract :
Rosmarinus officinalis L., a medicinal herb from the labiates family, has been reported
to have potential benefit in the treatment and prevention of several diseases. In particular its
phenolics have demonstrated protective effects on various types of cancer through several
mechanisms. The present study aimed to determine the effects of rosemary phenolic extracts
on human cell functions, with particular regard to their anti-proliferative properties in three
cell types U937, CaCo-2 and the peripheral blood mononuclear cells (PBMCs). The radical
scavenging and Ferric reducing abilities of the extracts have been assessed as well as their
cyto-toxicity and effects on cell cycle distribution and apoptosis. About 13 compounds
were identified with dominance of rosmarinic acid in the methanolic extract and phenolic
diterpens in the ethyl acetate fraction (Carnosol, Carnosic acid and methyl Carnosate). The
total polyphenolic content was important in the first extract with 2.589 ± 0.005 g/100 g in
gallic acid equivalent compared to 0.763 ± 0.005 g/100 g. The methanolic fraction displayed
higher antioxidant activity (DPPHIC50: 0.510 mg/mL and FRAP: 1.714 ± 0.068 mmol Fe2+/g)
while ethyl acetate showed pronounced antiproliferative effects (IC50: 14.85 ± 0.20μg/mL and
14.95 ± 2.32 μg/mL respectively for U937 and CaCo-2 cells). The anti-proliferative effect was
associated with a cell cycle arrest in S phase for U937 (62% of the population at 5 μg/mL) with
a concomitant decrease in G1 and G2/M phases. Tested extracts displayed in addition early
apoptotic effects in U937 and late apoptosis in CaCo-2 cells. The obtained data indicate that the
identified phenolics are at least partially responsible for the observed cytotoxicity.
Keywords :
Rosemary extracts , Cell cycle , Apoptosis , Antiproliferative activity , Antioxidant activity
