Author/Authors :
Ting, Zhao School of Chemistry and Chemical Engineering - Jiangsu University, Zhenjiang 212013, P.R.China , Yina, Fan School of Pharmacy - Jiangsu University, Zhenjiang 212013, P.R. China , Guanghua, Mao School of Food and Biological Engineering - Jiangsu University, Zhenjiang 212013, P.R.China , Yanmin, Zou School of Environment and safety - Jiangsu University, Zhenjiang 212013, P.R.China , Ye, Zou School of Food and Biological Engineering - Jiangsu University, Zhenjiang 212013, P.R.China , Liuqing, Yang School of Chemistry and Chemical Engineering - Jiangsu University, Zhenjiang 212013, P.R.China , Xiangyang, Wu School of Environment and safety - Jiangsu University, Zhenjiang 212013, P.R.China
Abstract :
Our previous study revealed that the antioxidant activity of polysaccharide (coded as
FGFP) extracted from Grifola frondosa by enzymolysis treatment was significantly superior
than that (coded as GFP) extracted by boiling-water. In this study, one purified polysaccharide
fractions (coded as FGFP-11) was obtained from FGFP by purified using DEAE-52 column and
Sephacryl S-500HR column. Results indicated that FGFP-11 with MW of 59.82 kDa consisted
of mannose, glucose and galactose with a molar ratio of 1.00:16.36:5.25. Fourier Transform
Infrared Spectroscopy (FTIR spectrum) of FGFP-11 was similar with that of polysaccharide
extracted by boiling-water from Grifola frondosa. These indicated the enzymolysis did not
destroy the polysaccharide structure. NMR spectrum showed that FGFP-11 possess α-(1→6)
glycosidic bond and α-(1→3) glycosidic bond configuration. The experiment of Congo red
also revealed that FGFP-11 had triple helix stereo-configuration. Moreover, the antioxidant
activities of FGFP-11 were improved compared with that of GFP, especially in scavenging of
hydroxyl radical and diphenyl picryl hydrazinyl (DPPH) radical.
Keywords :
Antioxidant Activities , Characterization , Purification , Enzymolysis , Polysaccharide from Grifola frondosa
