Author/Authors :
Wang, Yancui College of Life Sciences - Northwest A & F University, Yangling, Shaanxi 712100, China , Liu, Feng School of Pharmacy - Health Science Center - Xi›an Jiaotong University, Xi’an, Shaanxi Province, China , Peng, Liang College of Life Sciences - Northwest A & F University, Yangling, Shaanxi 712100, China , Wang, Bangqing College of Life Sciences - Northwest A & F University, Yangling, Shaanxi 712100, China , Yu, Jing College of Life Sciences - Northwest A & F University, Yangling, Shaanxi 712100, China , Su, Yingying Shaanxi Buchang Pharmaceutical Co, Ltd, Shaanxi, China , Ma, Cunde Shaanxi Buchang Pharmaceutical Co, Ltd, Shaanxi, China
Abstract :
The root of Ophiopogon japonicus has been used as a traditional Chinese medicine and
also a functional food ingredient for a long time in China. In the present study, 17 different
homoisoflavonoid compounds were identified in the root extract of O. japonicus by HPLC–
DAD and LCMS/MS analyses. The antioxidant activity of the of chloroform/methanol (1:1,
v/v), methanol and 70% ethanol extracts, and two major isolated homoisoflavonoid compounds
(methylophiopogonanone A and methylophiopogonanone B) from O. japonicus root were
investigated by various in-vitro assays. Methylophiopogonanone B showed the highest
antioxidant ability according to four antioxidant methods. Among the extracts, the chloroform/
methanol extract which contained high amounts of homoisoflavonoids was found to exhibit the
strongest antioxidant activity. The results showed that O. japonicus root can be regarded as a
potential source of homoisoflavonoids and natural antioxidant.
Keywords :
Antioxidant activity , LCMS/MS analysis , Homoisoflavonoids , Ophiopogon japonicus
