Author/Authors :
Lim, Wen Huei aAdvanced Oleochemical Technology Division (AOTD), Malaysia Palm Oil Board (MPOB), Malaysia , Tan, Yann Jean School of Postgraduate Studies and Research - International Medical University, 57000 Kuala Lumpur, Malaysia , Lee, Choy Sin Department of Pharmaceutical Chemistry - School of Pharmacy - International Medical University, 57000 Kuala Lumpur, Malaysia , Er, Hui Meng Department of Pharmaceutical Chemistry - School of Pharmacy - International Medical University, 57000 Kuala Lumpur, Malaysia , Wong, Shew Fung Department of Pathology - School of Medicine - International Medical University, 57000 Kuala Lumpur, Malaysia
Abstract :
Palm-based lipid nanoparticle formulation loaded with griseofulvin was prepared by solventfree
hot homogenization method. The griseofulvin loaded lipid nanoparticles were prepared
via stages of optimisation, by altering the high pressure homogenisation (HPH) parameters,
screening on palm-based lipids and Tween series surfactants and selection of lipid to surfactant
ratios. A HPLC method has been validated for the drug loading capacity study. The optimum
HPH parameter was determined to be 1500 bar with 5 cycles and among the palm-based
lipid materials; Lipid C (triglycerides) was selected for the preparation of lipid nanoparticles.
Tween 80 was chosen from the Tween series surfactants for its highest saturated solubility
of griseofulvin at 53.1 ± 2.16 μg/mL. The optimum formulation of the griseofulvin loaded
lipid nanoparticles demonstrated nano-range of particle size (179.8 nm) with intermediate
distribution index (PDI) of 0.306, zeta potential of -27.9 mV and drug loading of 0.77%. The
formulation was stable upon storage for 1 month at room temperature (25 °C) and 45 °C with
consistent drug loading capacity.
Keywords :
Zeta potential , Stability , Drug loading , Particle size , Griseofulvin , Palm , Lipid nanoparticles
