Author/Authors :
Sotoudegan, Farzaneh Department of Pharmaceutics - School of Pharmacy & Protein Technology Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Amini, Mohsen Department of Pharmaceutical Chemistry - Faculty of Pharmacy - Tehran University of Medical Sciences, Tehran, Iran , Faizi, Mehrdad Department of Pharmacology and Toxicology - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Aboofazeli, Reza Department of Pharmaceutics - School of Pharmacy & Protein Technology Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
A rapid, simple and reproducible HPLC method was developed and validated for the
analysis of nimodipine (NM) and/or its metabolite, oxidized nimodipine (OX–NM) in rat
cerebrospinal fluid (CSF) and artificial CSF. The NM and OX–NM were eluted in less than
10 min with no interferences from the endogenous CSF peaks. Analysis was carried out on a
Eurospher Performance (RP-C18, 250 × 4.6 mm) column and UV detection at 236 nm. The
mobile phase consisted of acetonitrile and water (70:30 v/v, respectively) with a flow rate of 1
mL/min. Limit of detection was 0.1 μg/mL for OX-NM. The calibration curve was linear over
the concentration range of 0.5-10 μg/mL and analytical recovery was more than 95%. The
coefficients of variation for intra-day and inter-day assays were less than 5%.
Keywords :
Polymeric micelle , Cerebrospinal fluid , HPLC , Nimodipine oxide , Nimodipine
