Author/Authors :
Ghasemi, Sainaz Department of Pharmacology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Salarian, Amir Ahmad Department of Toxicology - AJA University of Medical Science, Tehran, Iran , Zare Mirakabadi, Abbas Venomous Animals and Antivenom Production Department - Razi Vaccine and Serum Research Institute, Karaj, Iran , Jafarinejad, Somayeh Department of Pharmacology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Ghazi-Khansari, Mahmoud Department of Pharmacology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Scorpion venom toxicity is one of the major medical concerns from old years, due to its
influence on human activities and health. From many years ago a lot of researches established
to examine different aspects of venom toxicity and its effects on different organs. During these
years researchers are doing more specific studies on the cytotoxicity of scorpion venom. In
Iran, Odonthobuthus doriae, the yellow scorpion is one of the major threats based on its neuro
toxicity and severe pathophysiologic effects and researchers tried to find the mechanism of
these neuro toxic effects. The previous studies have shown that in isolated organs the yellow
scorpion venom is affecting the ion channels. Also some studies showed that this venom has
severe cytotoxic effects on the cell lines with many ion channels like nerve cell lines.
In this study, the cytotoxic effect of the crude venom of O.doriae on the 1321N1 cell line
(cancerous nerve cells) was studied. Primary cell cultured investigated in the presence of
different ion channel blockers: Ouabain (1mmol as Na channel blocker), Nifedipin (100 μmol
as Ca channel blocker), and TEA (40 mmol as K channel blocker) by MTT method. The result
showed that the O.doriae crude venom has cytotoxic effect via Na channels.
Keywords :
cell culture , Channel blocker , 1321N1 , Odonthobuthus doriae , Scorpion
